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OBJECTIVE:To systematically evaluate the effects of atorvastatin on pulmonary function,pulmonary arterial pres-sure and related indexes in patients with stable chronic obstructive pulmonary disease(COPD),and to provide evidence-based refer-ence. METHODS:Retrieved from Cochrane Library,PubMed,EMBase,CJFD and VIP,randomized controlled trials(RCTs)about atorvastatin combined with conventional therapy(trial group)vs. conventional therapy alone(control group)in the treatment of sta-ble COPD were collected. Meta-analysis was performed by using Rev Man 5.3 statistical software after data extraction and quality evaluation by Cochrane Handbook Manual 5.1.0. RESULTS:Totally 7 RCTs were included,involving 371 patients. Results of Me-ta-analysis showed,FEV1 [MD=0.07,95%CI(0.04,0.09),P<0.001],FEV1%pred [MD=6.18,95%CI(2.23,10.12),P=0.002] and 6MWD [MD=55.31,95%CI(36.44,74.18),P<0.001] of trial group were significantly higher/longer than those of con-trol group;pulmonary artery systolic pressure [MD=-6.78,95%CI(-11.62,-1.94),P=0.006],mean pulmonary artery pres-sure [MD=-6.61,95%CI(-7.26,-5.96),P<0.001],St. George respiratory questionnaire [MD=-13.21,95%CI(-23.90,-2.52), P=0.02] were significantly lower than control group,with statistical significance. There was no statistical difference in FEV1/FVC [MD=3.73,95%CI(-2.08,9.55),P=0.21] or hs-CRP [MD=0.29,95%CI(-1.37,1.95),P=0.73] between 2 groups. CONCLU-SIONS:Atorvastatin can significantly improve pulmonary function and pulmonary arterial pressure in patients with stable COPD, and can improve the quality of life.
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Objective To establish collagen‐induced arthritis (CIA) model used of DBA/1J mouse ,a preliminary study of the expression levels of BM Ps in mononuclear cells of peripheral blood and the joints tissue of CIA mice .Methods We induced DBA/1J mice and developed arthritis pathology by using of bovine type Ⅱ ;then ,we detected mRNA and protein expression levels of BMPs by using quantitative PCR and immunohistochemical staining .Results We successfully established CIA mouse model .The expres‐sion of BMP4 and BMP9 mRNA was significantly down‐regulated in peripheral blood mononuclear cells and in the pathogenesis of joint tissues of CIA mice (P<0 .05) .It showed that BMP9 protein significantly decreased in joint tissues of CIA mice by immuno‐fluorescence technique (P=0 .002) .Conclusion At the genetic level ,the expression of BMP4 and BMP9 could be significantly down‐regulated in the CIA mouse .At the protein level ,BMP9 could be significantly down‐regulated in the CIA mouse .
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<p><b>OBJECTIVE</b>To construct adenoviral vectors expressing mature miRNA-30a and miRNA-30e.</p><p><b>METHODS</b>The target mmu-miR-30a and mmu-miR-30e genes amplified from mouse genome were digested and linked to the shuttle plasmid pSES-HUS, which was then transformed into competent AdEaseier cells for recombination. The confirmed recombinant plasmids were transfected into Hek-293 cells for production of the adenoviruses pAd-mmu-miR-30a and pAd-mmu-miR-30e. The obtained adenoviruses were used to infect Mefs cells, and the cellular expressions of mmu-miR-30a and mmu-miR-30e were detected using fluorescence quantitative PCR.</p><p><b>RESULTS</b>mmu-miR-30a (357 bp) and mmu-miR-30e (324 bp) containing the restriction sites were amplified and linked to the shuttle plasmid pSES-HUS, which was successfully recombined with AdEasy1. After packaging in Hek-293 cells, the adenoviral vectors were obtained, which caused an increase of mmu-miR-30a expression by 26.46∓7.46 folds and mmu-miR-30e expression by 2.76∓0.25 folds in transfected Mefs cells.</p><p><b>CONCLUSION</b>We have successfully constructed the adenoviral vectors expressing the mature miRNAs.</p>
Subject(s)
Animals , Humans , Mice , Adenoviridae , Genetics , Base Sequence , Genetic Vectors , HEK293 Cells , MicroRNAs , Genetics , PlasmidsABSTRACT
OBJECTIVE@#To evaluate the efficacy and safety of combined treatment with angiotensin II receptor blocker (ARB) and angiotensin converting enzyme inhibitor (ACEI) on diabetic kidney disease.@*METHODS@#Randomized controlled trials (RCTs) were identified from CoChrane library, PubMed, EMbase, CNKI and VIP. Eleven RCTs involving 602 patients were included and analyzed with Rev Man 5.1 software.@*RESULTS@#Compared with ACEI alone, combined treatment with ARB and ACEI was more effective on decreasing 24 h albuminuria, systolic pressure, average 24 h systolic pressure, diastolic pressure, and average 24 h diastolic pressure but with a high level of serum potassium. Compared with ARB alone, combined treatment with ARB and ACEI was more effective on decreasing systolic pressure and diastolic pressure. Compared with ACEI or ARB alone, we didn't get a definite conclusion that whether combined treatment with ARB and ACEI was more effective on decreasing 24 h proteinuria.@*CONCLUSION@#Based on this Meta analysis, combined treatment with ARB and ACEI is safer and has positive effect on diabetic kidney disease. However, small sample size and low methodological quality appeared in most of the trials included in this systematic review. Therefore, available evidence is insufficient to recommend a routine clinical application of combined treatment with ARB and ACEI on diabetic kidney disease.
Subject(s)
Humans , Angiotensin Receptor Antagonists , Therapeutic Uses , Angiotensin-Converting Enzyme Inhibitors , Therapeutic Uses , Diabetic Nephropathies , Drug Therapy , Drug Therapy, Combination , Randomized Controlled Trials as TopicABSTRACT
0.05).?-defensin 2 was detected in the specimens of vocal cord polyp,but very little in the subjects of other two groups.Its expression level was significantly higher in the vocal cord polyp than that of the other two groups(P